Wednesday, November 27, 2019

Salvador Luria essays

Salvador Luria essays Salvador Luria was one of the founders of microbiology, as we know it. He emigrated from here from his native country of Italy in 1940. His work in the United States is his best known. His work on bacteriophage (bacterial virus) here brought up many new topics in bacteriology, biochemistry, and virology. Born in 1912 in Turin, Italy Salvador Luria was born to David Luria and Ester Sacerdote. His father was a well-respected Jewish leader in his hometown. Salvador attended Liceo dAzeglio high school. This was one of Northern Italys most highly recognized schools. After he finished high school he enrolled in medical school at the University of Turin. In medical school he studied with nerve tissue expert Giuseppe Levi. He met Ugo Fano who later taught him calculus and physics in an after school class using astronomy as a base. The influence that Fano had on Salvador was so great that he decided to pursue basic sciences. He decided to go with Radiology, he believed this was the gap between physics and medicine. He received his medical degree in 1935. Although he had received his degree he was not happy. He believed Radiology was the most boring part of the medical world. Salvador was drafted into the Italian Army as a medic. This proved he was not made for a medical career. He was discharged in 1937 and moved to Rome. In Rome he study at the Physics Institute of the University of Rome. He was shown the writings of Max Delbruck, who had boldly stated a gene, was a molecule. Salvador later said that Maxs writings were the Holy Grail of biophysics. While living in an old broken down trolley car in the streets of Rome Salvador started a conversation with a microbiologist by the name of Geo Rita. Geo introduced him to bacteriophage, Salvador believed he could prove Maxs theory. He fled Europe in 1940 when the Nazi war machine was an approaching. He acquired an American visa and came ...

Saturday, November 23, 2019

5 Part-Time Jobs That Pay Well

5 Part-Time Jobs That Pay Well What kind of job allows you to work less and yet earn more? While the question may sound like a riddle, it’s actually a reality when it comes to several high-paying part-time jobs. Let’s count down five of the most sought after part-time positions, along with how to score one. 1. Management AnalystAccording to figures from the Bureau of Labor Statistics, management analysts require only a bachelor’s degree, less than five years of experience, no specific on-the-job training and yet make an average of $37.79 per hour. Even better? Because most accountants work on a contractual basis, they can set their own hours.Also called management consultants, these professionals evaluate an organization’s efficiency and propose strategies to trim costs and increase revenues. While it’s not required, a Certified Management Consultant (CMC) can boost your appeal to potential employers. Additionally, prior work experience in a number of different fields - from hum an resources to information technology - can help you gain an inside edge.Not only is the pay high, but the demand is, as well: between now and 2022, the accounting profession is expected to grow by a significant 20 percent.2. Fitness InstructorFrom yoga to Zumba, fitness instructors lead individuals of all ages and skill levels as well as groups in any number of different fitness classes.While on paper the pay may not in itself be extraordinary - an average of $15.25 per hour - working as a fitness instructor requires no education beyond the high school diploma or its equivalent, although many fitness instructors do choose to pursue an associate’s or bachelor’s degree in a health or fitness related field, such as physical education, kinesiology, or exercise science.With a projected growth rate of 13 percent between now and 2022, job security for fitness instructors is promising. Not to mention the flexibility to work early morning and evening hours depending on you r individual scheduling needs.3. Graphic DesignerGraphic designers communicate ideas through visual concepts and help with layout and production design for a variety of print materials. In 2012, nearly 25 percent of graphic designers were self-employed which not only lets them have key input as to how many hours they work but also when and where they work them.A bachelor’s degree in graphic design or a related field is typically expected for a career in this field, along with an aptitude for the creative arts. The average haul of a graphic designer in 2015? $21.22 per hour.4. AccountantWhile most accountants work full-time, many accounting specialists are finding part-time jobs to be particularly appealing. In fact, a recent survey of chief financial officers revealed that 72 percent of CFOs use temporary staffing solutions to help reach their accounting goals.Most accountants have a minimum of a bachelor’s degree in accounting or a related field; becoming a Certified Public Accountant (CPA) further improves job prospects. Not only do part-time accountants earn a competitive compensation of an average of $30.55 per hour, but some even receive employee benefits.The ability to work part-time also allows unparalleled flexibility that is particularly appealing to everyone from students to people nearing retirement.5. Computer ProgrammerComputer programmers create software programs by writing code for an average hourly pay of $35.71 per hour. Most computer programmers have at least a bachelor’s degree, although an associate’s degree may suffice. However, because the field changes so rapidly, programmers are expected to keep up with its evolution through continuing education and professional development course.One benefit enjoyed by computer programmers? The ability to telecommute from anywhere in the world.Whether you’re simply in need of extra cash, looking for a change of pace, or require the flexibility of part-time work, these gigs offer high returns for workers in these fields. Looking for an inside edge on getting the part-time job of your dreams? Sign up for a job match alert.

Thursday, November 21, 2019

Writing a protocol for genomic DNA extraction and PCR Lab Report

Writing a protocol for genomic DNA extraction and PCR - Lab Report Example blood collection for control experiment was equally undertaken for the purpose with regards to the manufactures protocol . the sampling of the genomic DNA was used as templates for the PCR amplification with specific T .cruzi Mdna primers . Tcz1 and TC z2 and the KDNA primers s35 and s36 . the amplification reaction was run with 200ng templates DNA UNDER the subsequent conditions.0.2 Mm of each primer , 2.5 U Taq DNA Polymerase , 0.2 mM dNTP and 2 mM Mg Cl2 in a 25 ml final volume . the triplicate amplification reaction were performed using the recommendations temperatures for Ndna ( 950C for five minutes , 30 cycles of 950 C for 30 s , 620C for 1 min nd 72 0C for 1 min and final extension at 72 o C for 5 min ) . The amplicons were resolved in the 1.3 % agarose gel The tumor tissue average RFU 0.254 corresponds to 406.25 standard and controls tissue average RFU 0.197corresponds to 275 standards. The curve is gently rises in a direct proportional manner to huge figures. The scale of the graph presents 1 cm representing 125 ng/ul in y-axis while the 1 cm representing the 0.1 in x-axis (Maria & Frank , 10). 22. It is important to remove the supernatant then dry the pellet at the prevailing room temperature. Normally, the DNA do not attach or stick as expected on the walls of the tube following 70% ethanol wash. Caution and extreme should be maintained while to bid avoid any aspirating the pellet In conclusion, the protocol for DNA extraction and the PCR based typically on the main methods takes subsequent steps. In the process of extraction large quantities of the DNA may be necessitate heating briefly at 65oC) for the suspension. The resulting large great molecular weight may undergo several days for proper